Journal: Bioactive Materials
Article Title: One-step strategy for fabricating icariin-encapsulated biomimetic Scaffold: Orchestrating immune, angiogenic, and osteogenic cascade for enhanced bone regeneration
doi: 10.1016/j.bioactmat.2025.06.001
Figure Lengend Snippet: CHP@IC directs macrophage M2 polarization to regulate the immune microenvironment. (A–C) The FDA/PI staining, phalloidin staining, CCK8 result, and cell spread area of Raw264.7 cultured on the surface of CHP and CHP@IC scaffolds. (D) The RT-qPCR results of macrophage phenotypic transition at 5 days. (E)The WB results and the relative protein expression of iNOs, and ARG1 at 5 days. (F) The FC result of CD86 and CD206 at 5 days. (G) The IF staining of iNOs and CD206 at 5 days. (H) Quantitative analysis of iNOs, and CD206 fluorescent intensity (n = 3). (I) The RT-qPCR results of the cytokine in immune microenvironment at 5 days. (J) ELISA results of the cytokine at 5 days. (I) Schematic representation of the CHP@IC-mediated shift in the immune microenvironment.
Article Snippet: Then, the membrane was blocked with 5 % skimmed milk and incubated with iNOs (NB300-605, Novus, USA), ARG1 (16001-1-AP, Proteintech, UK), STAT3 (ET1607-38, HUABIO, China), phospho-STAT3 antibodies (S727) (ET1607-39, HUABIO, China) and GAPDH (ab8245, Abcam, UK) overnight.
Techniques: Staining, Cell Culture, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay